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Motor Neuron Development and Circuitry

We are studying how the neural circuitry that is required for coordinated walking in adult flies is constructed during development, and how it functions in the adult.

Recent Publication

Types of changes to CRM activities after random mutagenesis

Dense and pleiotropic regulatory information in a developmental enhancer

Timothy Fuqua, Jeff Jordan, Maria Elize van Breugel, Aliaksandr Halavatyi, Christian Tischer, Peter Polidoro, Namiko Abe, Albert Tsai, Richard S. Mann, David L. Stern & Justin Crocker

News

October 28, 2020

Enhancer Fracking

Check out our newest collaborative paper with the Stern and Crocker labs, where a CRM was randomly mutagenized to death and the results analyzed in vivo using a high-throughput robotics platform.

Fuqua, T., Jordan, J., van Breugel, M.E. et al. Dense and pleiotropic regulatory information in a developmental enhancer. Nature (2020). https://doi.org/10.1038/s41586-020-2816-5

February 12, 2020

New paper: Selective loss TF-DNA complex binding as a consequence of compromising DNA shape recognition

In this paper, Judith Kribelbauer, who was a joint student with Harmen Bussemaker, significantly extended our understanding of how homeodomain protein complexes (Exd-Hox) bind to the correct binding sites in vivo. Judith generated an amazing amount of data for this paper: high-throughput SELEX-seq assays on wild type and mutant homeodomain proteins, ChIP-seq analyses of tagged homeodomain proteins from wing imaginal discs, and Hi-C data from wing discs to identify enhancer-promoter interactions.